Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM150

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产品名称: Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM150

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Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM150

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Product Details
Product Name	Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM150]
Product Description	Rabbit monoclonal to Monomethylated Histone H3 Lysine 9, H3K9me1, Histone H3 (monomethyl K9)
Catalog No.	31-1064-00
Clone Name	RM150
Product Data Sheet	Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM150
Specificity	RM150 reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.
Immunogen	A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys9).
Application	Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.2 ug/mL-1 ug/mL; ChIP: 2 ug/mL-10 ug/mL ICC: 0.5 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
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Product Images
	RM150 specifically reacts to Histone H3 monomethy-lated at Lysine 9 (K9me1). No cross reactivity with non- modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM150 at 0.5 ug/mL, showed a band of histone H3 monomethy-lated at Lysine 9 (K9me1) in HeLa cells.
	ChIP performed on HeLa cells using H3K9me1 antibody (RM150, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
	Immunocytochemistry of HeLa cells treated withsodium butyrate, using Monomethyl-Histone H3(Lys9) Rabbit mAb RM150 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).

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