RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with mono- methylated Lysine 4 (K4me1), trimethylated Lysine 4(K4me3), or other methylations in histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM135 at 0.025 ug/mL, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells.
Immunocytochemistry of HeLa cells treated withsodium butyrate, using Dimethyl-Histone H3 (Lys4) Rabbit mAb RM135 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
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