Anti-Histone H3 (Unmodifed Lys4) Rabbit Monoclonal Antibody, Clone RM186

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产品名称: Anti-Histone H3 (Unmodifed Lys4) Rabbit Monoclonal Antibody, Clone RM186

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Anti-Histone H3 (Unmodifed Lys4) Rabbit Monoclonal Antibody, Clone RM186

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Product Details
Product Name	Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [RM186]
Product Description	Rabbit Monoclonal to Histone H3 (Unmodified Lys4); Histone H3 (Unmodified Lysine 4)
Catalog No.	31-1086-00
Clone Name	RM186
Product Data Sheet	Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody Data Sheet RM186
Specificity	RM186 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.
Immunogen	An unmodified peptide corresponding to the N-terminus of Histone H3.
Application	Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC)
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 1 ug/mL – 2 ug/mL; ELISA: 0.5 ug/mL - 1 ug/mL; Multiplex: 0.5 ug/mL – 2 ug/mL; Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
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Product Images
	RM186 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono- methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
	Western Blot of acid extracts of HeLa cells treated withsodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of histone H3 with unmodified Lys4.
	Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody (clone RM186) (red). Actin filaments have been labeled with fluorescein phalloidin (green).

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