RM156 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetyl- ated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14(K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM156 at 1.0 ug/mL, showed a band of histone H3 acetylated at Lysine 79 in treated HeLa.
Sandwich ELISA against acetylated histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3K79ac (RM156, 5 ug/mL) as the capture antibody and biotinylated anti-H3CT (RM188, 1 ug/mL) as the detection antibody.
ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K79Ac antibody (RM156, 5ug). Real-time PCR was performed using primers specific to the geneindicated.
Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys79) Rabbit mAb RM156 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
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