Western Blot of (1) acid extracts of HeLa cells treated withsodium butyrate and (2) recombinant histone H3.3, usingRM179 at 1 μg/mL, showed a band of histone H3 acetylatedat Lysine 56 in treated HeLa.
RM179 specifically reacts to Histone H3 acetylated at Lysine 56(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys56) Rabbit mAb RM179 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
Immunohistochemistry staining of HepG2 cells usingAnti-Acetyl-Histone H3 (Lys56) antibody, RM179.
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