RM154 specifically reacts to Histone H3 acetylated at Lysine 36 (K36ac), and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM154 at 1.0 ug/mL, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa.
Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys36) Rabbit mAb RM154 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
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