RM172 specifically reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM172 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa.
Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys27) Rabbit mAb RM172 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
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