RM130 specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.
Western Blot of acid extracts from HeLa cellsuntreated (-) or treated with sodium butyrate (+) or, using RM130 at 0.5 ug/mL.
ChIP performed on HeLa cells using H3K14ac antibody (RM130, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys14) Rabbit mAb RM130 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
本公司网站所展示销售的产品仅供科研!
沪公网安备 31011202007337号