RM149 specifically reacts to Histone H3 acetylatedat Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) inhistone H3.
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM149 at 0.5 ug/mL, showed a band of histone H3 acetylated at Lysine 4 in treated HeLa.
Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys4) Rabbit mAb RM149 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
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