RM260 specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetyl- ated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-mordified Lysine 23 in histone H2B.
Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using Acetyl-Histone H2B (Lys23) rabbit monoclonal antibody (RM260) at 0.5 ug/mL.
Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H2B (Lys23) rabbit monoclonal antibody (RM260) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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