Western Blot of acid extracts of HeLa cells treatedor non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylatedat serine 139 in HeLa cells.
Immunocytochemistry of HeLa cells using gammaH2AX Rabbit mAb RM224 (red). Actin filamentshave been labeled with fluorescein phalloidin (green).
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