RM221 specifically reacts to Histone H2A.Z acetylatedat Lysine 4 (K4ac). No cross reactivity with non-modifiedLysine 4 or other acetylated Lysines in histone H2A.
Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate,using RM221 at 0.5 ug/mL, showed a band ofhistone H2A.Z acetylated at Lysine 4 in treatedHeLa.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone RM221) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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