Western Blot of lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated. Using Anti-Phospho-Akt (Ser473) RM251 at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Akt (Ser473) RM251at a 1:400 dilution.
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