Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody, Clone RM263

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产品名称: Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody, Clone RM263

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Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody, Clone RM263

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Product Details
Product Name	Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody [RM263]
Product Description	Rabbit monoclonal to Histone H3.3 G34W; Histone H3.3 G34W Mutant
Catalog No.	31-1145-00
Clone Name	RM263
Product Data Sheet	Anti-Histone H3.3 G34W Mutant Rabbit Monoclonal Antibody Data Sheet RM263
Specificity	RM263 reacts to the Histone H3.3 G34W mutant. No cross reactivity with wild type Histone H3.3 .
Immunogen	A peptide corresponding to Histone H3.3 G34W mutant
Application	Western Blot ELISA Immunohistochemistry Immunocytochemistry Chromatin IP
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.5 ug/mL - 2 ug/mL; Immunohistochemistry (IHC): 0.5 – 5 ug/mL; Immunocytochemistry (ICC): 0.5 – 5 ug/mL.
References for Histone H3.3 G34W Mutant antibody [RM263]	Yamamoto H.et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017)10.1016/j.humpath.2017.11.020 IHC; Human. Read more (PubMed: 29241742) Kato, I. et alGiant Cell Tumours of Bone Treated with Denosumab: Histologic, Immunohistochemical, and H3F3A Mutation AnalysesHistopathology 10.1111/his.13448.(2017). IHC-P; Human. IF; Human Read more (PubMed: 29206281) Luke J. et al. H3F3A mutation in giant cell tumour of the bone is detected by immunohistochemistry using a monoclonal antibody against the G34W mutated site of the histone H3.3 variant.Histopathology 10.1111/his.13190.(2017). IHC-P; Human. Read more (PubMed: 28211081) Amary F. et al. H3F3A (Histone 3.3) G34W Immunohistochemistry: A Reliable Marker Defining Benign and Malignant Giant Cell Tumor of Bone.AM J Surg Pathol.10.1097/PAS.0000000000000859. (2017). IHC-P; Human. Read more (PubMed: 28505000)
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Product Images
	Western Blot analysis of cell lysates prepared from293T, transfected with a DNA construct encodingG34W mutant or wild type proteins of Histone H3.3, using anti-Histone H3.3 G34W clone RM263.
	Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNAconstruct encoding Histone H3.3 G34W mutant or wildtype, stained with anti-Histone H3.3 G34W clone RM263.
	Immunohistochemical staining of Giant Cell Bone Tumor (GCBT) tissue sections using Anti-Histone H3.3 G34W antibody RM263. Image courtesy of Adrienne Flanagan, Department of Histopathology, RONH, Stanmore, Middlesex, UK
	Immunohistochemical staining of Giant Cell Bone Tumor (GCBT) tissue sections using Anti-Histone H3.3 G34W antibody RM263. Image courtesy of Adrienne Flanagan, Department of Histopathology, RONH, Stanmore, Middlesex, UK

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