Anti-His-Tag Chimeric Human Monoclonal Antibody, Clone RMH01

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产品名称: Anti-His-Tag Chimeric Human Monoclonal Antibody, Clone RMH01

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Anti-His-Tag Chimeric Human Monoclonal Antibody, Clone RMH01

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Product Details
Product Name	Anti-His-Tag Chimeric Human Monoclonal Antibody, [RMH01]
Product Description	Chimeric Human monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy) This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
Catalog No.	54-1161-00
Clone Name	RMH01
Product Data Sheet	Anti-His-Tag Chimeric Human Monoclonal Antibody Data Sheet RMH01
Specificity	RMH01 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein in mammalian or bacteria cells.
Immunogen	Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Application	Western Blot ELISA (Capturer or Detector)
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: Capturer: 0. 1 ug/mL – 2 ug/mL; Detector: 0.01 ug/mL – 0.5 ug/mL; Western Blot: 0.1 ug/mL – 1 ug/mL.
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Product Images
	Sandwich ELISA performed on different amounts ofwhole lysate of 293T cells transfected with a DNA construct encoding HisTag Histone H3 (K36M) protein, using 50uL/well of different concentrations of chimeric human anti-HisTag antibody Clone RMH01 as the capture antibody. Anti-H3K36M rabbit monoclonal antibody (RM193) was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-http://www.revmab.com/wp-content/uploads/2016/10/54-1161-00-Human-Chimeric-Histag-x6-x10_RMH01.pdfRabbit IgG as the Secondary antibody.
	A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of chimeric human anti-HisTag antibody Clone RMH01 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.
	Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.

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