BRAF V600E Rabbit Monoclonal Antibody, Clone RM8

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产品名称: BRAF V600E Rabbit Monoclonal Antibody, Clone RM8

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BRAF V600E Rabbit Monoclonal Antibody, Clone RM8

BRAF V600E Rabbit Monoclonal Antibody, Clone RM8 的详细介绍

Product Details
Product Name	BRAF V600E Rabbit Monoclonal Antibody [RM8]
Product Description	Rabbit monoclonal to BRAF V600E Mutant, BRAF(V600E), BRAF-V600E
Catalog No.	31-1042-00
Clone Name	RM8
Product Data Sheet	BRAF V600E Rabbit Monoclonal Antibody Data Sheet
Specificity	RM8 reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
Immunogen	A peptide corresponding to BRAF V600E mutant
Application	Immunohistochemistry Immunocytochemistry Western Blot
Species Reactivity	Human
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	100ug
Concentration	1 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Immunohistochemistry (IHC): 0.5 – 5 ug/mL; Immunocytochemistry (ICC): 2 – 10 ug/mL; Western Blot (WB): 0.5 – 2 ug/mL
References for BRAF V600E Mutant antibody [RM8]	Sun HL et al. ERK Activation Globally Downregulates miRNAs through Phosphorylating Exportin-5. Cancer Cell 10.1016/j.ccell.2016.10.001. WB; Human. Read more (PubMed: 27846390) Yang Het al. Activated MEK cooperates with Cdkn2a and Pten loss to promote the development and maintenance of melanoma. Oncogene 10.1038/onc.2016.526. WB; Human. Read more (PubMed: 28263969)
Protocols	BRAF V600E IHC Protocol
Product Images
	Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections.
	Immunohistochemical staining of formalin fixed andparaffin embedded Thyroid Carcinoma tissue. Allele specific PCR validated to be Negative for BRAF V600E.
	Immunohistochemical staining of formalin fixed andparaffin embedded Thyroid Carcinoma tissue. Allele specific PCR validated to be Positive for BRAF V600E.
	Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein.
	Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. WiDr Cells (400X, Positive)
	Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. SW40 Cells (400X, Negative)

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