Anti-HA-Tag Chimeric Human Monoclonal Antibody, Clone RMH02

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产品名称: Anti-HA-Tag Chimeric Human Monoclonal Antibody, Clone RMH02

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Anti-HA-Tag Chimeric Human Monoclonal Antibody, Clone RMH02

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Product Details
Product Name	Anti-HA-Tag Chimeric Human Monoclonal Antibody, [RMH02]
Product Description	Chimeric Human monoclonal to HA-Tag This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HA-Tag monoclonal antibody (RM305), with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody.
Catalog No.	54-1191-00
Clone Name	RMH02
Product Data Sheet	Anti-HA-Tag Chimeric Human Monoclonal Antibody Data Sheet RMH02
Specificity	RMH02 reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.
Immunogen	A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106).
Application	Western Blot ELISA (Capturer or Detector)
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: Capturer: 0. 5 ug/mL – 2 ug/mL; Detector: 0.01 ug/mL – 0.5 ug/mL; Western Blot: 0.1 ug/mL – 1 ug/mL.
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Product Images
	Sandwich ELISA performed on whole lysate of LNCaP cells overexpressing HA-tag Bag1 protein. ELISA plate was coated with 50uL/well of chimeric human anti-HA- Tag antibody Clone RMH02 (1ug/mL) as the capture antibody. Anti-Bag1 rabbit serum was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.
	Western blot of 293T cells transfected (+) or non- transfected (-) with a DNA construct encoding HA- Tag KRas fusion protein, using anti-HA-tag rabbit monoclonal antibody (clone RM305) at 0.1 ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.

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