H3K4me3 Mouse Monoclonal Antibody
Clonality: Mouse IG1
Source: Mouse
Applications: WB: 1:500-1:2000
Cross-Reactivity: H MRat
Concentration:100 ug/100 ul
Detected band (by western) MW: 15 KD
Alt. Names
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Specificity:
The Histone H3 (tri methyl K4) mouse monoclonal antibody can recognize endogenous Histone H3 (tri methyl K4) proteins.
Immunogen:
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
Storage Condition and Buffer:
Store at -20°C. Stable for one year from the date of shipment. 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7 with Histone H3 (tri methyl K4) Mouse mAb diluted at 1:1000.
H3K4 methylation is one of the gene activation markers and one of the hottest epigenetic targets in recent studies. H3K4me1, H3K4me2 and H3K4me3 have a commonality for gene activation, although their subsets play distinct roles in modulating chromatin function (4). Among which, H3K4me3 occupies as many as 75% of all human gene promoters in several cell types (e.g., ES cells), playing important role in mammalian gene expression (1). H3K4me2 decorates genomic regions independently of H3K4me3, although most of it overlaps with H3K4me3 near the transcription start sites (2). Monomethylated H3K4 (H3K4me1) also co-occupies regions near the start sites with H3K4me3. Apart from the transcription start site, H3K4me1, together with H2K27 acetylation, specifies enhancer regions (3).
In recent studies, H3K4 has been demonstrated to be related stem cell self-renewal and differentiation (4).
H3K4 methylation statuses can be detected by our H3K4 methylation antibody series.
References:
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