Oropouche Virus Gn protein – Human Fc

Oropouche virus (OROV) is an RNA virus that belongs to the family Bunyaviridae. OROV can cause an acute febrile illness, with other symptoms similar to those reported for Dengue, Zika, and Chikungunya virus infection, including headache, myalgia, joint pain, nausea and vomiting. In some cases, OROV can cause a type of viral meningitis.

In humans, OROV is transmitted by the biting midge culicoides paraensis, an insect vector that lives in urban areas and is widely distributed in South America. Since the virus was first isolated in 1955, OROV has caused 30 large scale epidemics in the Amazon region infecting at least 500,000 people. The incidence of Oropouche fever has now become widespread in Latin American countries where c.paraensis is present.

Differential diagnosis of Oropouche viral infection from infection with Dengue, Zika or Chikungunya is recognised to be important, but there are few diagnostic assays available. The Native Antigen Company provides a range of Oropouche virus antigens to facilitate research into the virus, and to support immunoassay develpment.

Oropouche virus Gn protein is a type I integral transmembrane protein, which is further modified by N-linked glycosylation. Together with Gc protein it forms spikes on the virus particle and is essential for viral attachment and cell fusion. It has been described that for certain bunyaviruses, Gn and Gc are targeted by neutralizing antibodies

Oropouche virus Gn protein was expressed in mammalian HEK293 cells. The transmembrane region of the protein has been replaced with human IgG1 Fc, via a glycine-serine linker. The purified protein is approximately 55kD on analysis by reducing SDS-PAGE.

References:

Tilston-Lunel, N.L. (2016) J. Virol. 90:2616