iLite® GM-CSF Assay Ready Cells are a genetically engineered reporter gene cell line (U937, ATCC# CRL1593.2) responsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.
>250 µL of iLite® Assay Ready Cells suspended in RPMI 1640 with 20% FBS, mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).
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