Other

LCN2

NGAL, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25

Homo sapiens (Human)

Full Length of Mature Protein

8060329; 9339356; 14702039; 15164053; 15489334; 7835423; 7683678; 1281792; 12453413; 15084671; 16335952; 16740002; 19229297; 19159218; 21269460; 10684642; 10339412; 12453412

41.0 kDa

Tag-Free

E.Coli

>95% as determined by SDS-PAGE and HPLC.

0.2 μm filtered 20 mM PB, pH 7.4, 150mM NaCl ,lyophilized

Less than 1.0 EU/μg as determined by LAL method.

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x10⁶ IU/mg.

NGAL_HUMAN

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. {ECO:0000269|PubMed:12453413}.

QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG

21-198aa

Cancer