Turbocharged Protease Inhibitor Cocktail is a broad spectrum Protease Inhibitor cocktail has been specifically designed to provide complete protection of valuable proteins of interest from degradation during extraction and purification.
Features and Benefits: Complete protection from proteases in one single cocktail.
All our cocktails contain AEBSF, the preferred serine protease inhibitor, to provide superior protection with unmatched stability, convenience and reliability.
The cocktail formulations are available with or without EDTA which may interfere with protein stability or certain downstream applications.
Our cocktails are lyophilized solids which dissolve instantaneously making them readily available for their instantaneous inhibitory action and are easier to use than tablets that require splitting, vortexing and time consuming dissolution.
Our lyophilized solids are guaranteed for stability, reproducibility and have a shelf life of over a year. Most other commercially available cocktails made and sold in aqueous solutions offer very low guarantee of stability after 1 month of being manufactured (Lit ref: Bestatin, Leupeptin solution in water are stable for 1 month at -20oC).
Economical: More cost effective than other commercially available formulations.
Reliable: Count on our expertise in the production of high quality cocktails that have been successfully used for 20 + years by thousands of researchers worldwide.
Product Description: White Lyophilized Solid. The cocktail contains 8 inhibitors targeting against the major classes of proteases as outlined in the following table.
Products
Target
Target Proteases
AEBSF, HCl
Serine Proteases
Leupeptin
Cysteine Proteases and Trypsin-like Proteases
Aprotinin
Broad Spectrum, Serine Proteases
Pepstatin A
Aspartic Proteases
Bestatin
Aminopeptidase B and Leucine Aminopeptidase
EDTA
Metalloproteases
Metalloendopeptidases
E-64
Cysteine Proteases
Antipain
Papain, Cathepsin A/B
Performance Activity Assay:Comparison of our Turbocharged Protease and Phosphatase Inhibitor Cocktails with Competitor’s commercial tablets.
Figure 1: Using a general protease activity assay and pancreas extract, our Turbocharged Cocktails were tested against Competitor’s commercially available tablets under the same conditions. The results were based on protease activity in the absence of any inhibitor cocktails (Test 1); compared to protease activity in the presence of Competitors Protease Inhibitor Tablets (Test 2); Turbocharged Protease Inhibitor Cocktail (T-2492/Test 3); Turbocharged Protease + Phosphatase Inhibitor Cocktail (T-2495/Test 4); and Blank
Figure 2: Using a general phosphatase activity assay the alkaline phosphatase activity was determined with no inhibitor cocktail added (Test 1); compared to Phosphatase activity in the presence of Turbocharged Phosphatase Inhibitor Cocktail (T-2494/Test 2); Turbocharged Protease + Phosphatase Inhibitor Cocktail (T-2495/Test 3); and Blank
The results indicate that our Turbocharged Cocktails showed better performance ( > 97% inhibition) than the Competitors product with our Turbocharged Protease + Phosphatase inhibitor Cocktails being the only one of its kind available in the market today inhibiting both Proteases + Phosphatases available as a lyophilized solid in a single vial.
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