This broad spectrum Phosphatase Inhibitor cocktail has been specifically designed to provide complete protection of valuable proteins of interest from degradation during extraction and purification.
Features and Benefits: Complete protection from phosphatases in one single cocktail.
Product Description: White Lyophilized Solid. The cocktail contains 8 inhibitors targeting against the major classes of phosphatases as outlined in the following table
Products
Target Phosphatases
Imidazole
Alkaline Phosphatases
Sodium Pyrophosphate
PP1 and PP2A
Sodium Orthovanadate
Protein Tyrosine Phosphatase, Alkaline Phosphatase
Glycerophosphate
Ser/Thr Phosphatases
Sodium Molybdate
Acid and Phosphoprotein Phosphatases
Na Tartrate Dihydrate
Acid Phosphatases
Na F
Bromotetramisole oxalate
Recommended Usage: This cocktail has been optimized for effectiveness, convenience, stability, reproducibility and has been tested for general use. It inhibits activity in extracts from almost any tissue or cell types including animals, plants, yeasts, bacteria and fungi.
Reconstitute each vial with 1 ml of water to obtain a concentrated stock solution. Dilute this conc stock solution to 1X before use. Recommended dilution is 1:100 (based on the application). One vial is recommended for 10g-20g of tissue or cell extract.
Performance Activity Assay: Comparison of our Turbocharged Protease and Phosphatase Inhibitor Cocktails with Competitor’s commercial tablets.
Figure 1: Using a general protease activity assay and pancreas extract, our Turbocharged Cocktails were tested against Competitor’s commercially available tablets under the same conditions. The results were based on protease activity in the absence of any inhibitor cocktails (Test 1); compared to protease activity in the presence of Competitors Protease Inhibitor Tablets (Test 2); Turbocharged Protease Inhibitor Cocktail (T-2492/Test 3); Turbocharged Protease + Phosphatase Inhibitor Cocktail (T-2495/Test 4); and Blank
Figure 2: Using a general phosphatase activity assay the alkaline phosphatase activity was determined with no inhibitor cocktail added (Test 1); compared to Phosphatase activity in the presence of Turbocharged Phosphatase Inhibitor Cocktail (T-2494/Test 2); Turbocharged Protease + Phosphatase Inhibitor Cocktail (T-2495/Test 3); and Blank
The results indicate that our Turbocharged Cocktails showed better performance ( > 97% inhibition) than the Competitors product with our Turbocharged Protease + Phosphatase inhibitor Cocktails being the only one of its kind available in the market today inhibiting both Proteases + Phosphatases available as a lyophilized solid in a single vial.
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