Applications: Tosyl-activated 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through reduced disulfide bounds in the hinge region is a popular way to couple without interfering with the antigen binding region.
Technical specifications:
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