Protein G (Agarose) 4B-CL

Applications:

• Protein G binds specifically to F_c regions of many mammalian immunoglobulins.
• Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids.
• These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments.
• Affinity matrix with immobilized protein G (from Streptococcus sp.) by CNBr activation coupling method.
• Protein G- 4B-CL is used for purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.

Technical Specifications

• Ligand: Native Protein-G from Streptococcus sp.
• Matrix: 4B-CL (crosslinked agarose beads, 4%)
• Matrix activation: Cyanogen bromide
• Particle size range: 52 – 165 µm
• Exclusion limit range: 6 x 10⁴ - 2 x 10⁷
• Ligand density ~ 2 mg Protein-G / ml drained medium 
• Binding capacity: ~ 20 mg human IgG / ml drained gel
• pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
• Storage: 2 – 8 °C
• Supplied as suspension in 0.5M NaCl

Additional Information

Additional Information

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