Octyl (Agarose) 4B-CL

Applications: Octyl- 4B-CL is a hydrophobic chromatorgraphy matrix used to separate proteins on the basis of relative hydrophobicity. This is particularly useful for the purification from high ionic strength biological extracts since binding is performed in the presence of salt and the elution is carried out in the absence of salt.

Technical specifications:

• Active group: Octyl group
• Matrix: 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 μm
• Molecular weight range: 6 x 10⁴ – 2 x 10⁷
• Matrix activation: Epoxy
• Matrix attachment: Ether
• Matrix spacer: 12 atoms
• Binding capacity: 3 – 5 mg β-lactoglobulin / ml medium and 5-10 mg human serum albumin / ml drained gel
• pH stability: 4 – 9
• Flow specifications: 70 – 140 cm / h
• Chemical stability: Stable in common buffers, chaotropic agents, detergents, and polar organic solvents
• Supplied as a suspension in 0.5M NaCl containing 0.02% thimerosal
• Regeneration: 1M NaOH; 8M urea

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