Inhibitor of HSP90. Novobiocin interacts with the C-terminal ATP-binding domain of HSP90, unlike 17-AAG (A1256), Geldanamycin (G1047), Herbimycin A (H1050), or Radicicol (R1130) which all bind to the N-terminal ATP- of HSP90. Also useful in production of positively supercoiled plasma DNA, targeting the nucleotide- of gyrase B.
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