Maltose (Agarose) 4B

Applications:

Maltose is coupled with 4B. Maltose 4B provides a convenient affinity purification of recombinant proteins tagged with maltose-binding protein (MBP). Maltose has high binding capacity for MBP-tagged proteins. Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step.

Maltose- 4B is used in protein chromatography, affinity chromatography and carbohydrate matrices. Maltose- 4B has been used to study surfactant protein D (SP-D), which plays an important role in innate immunity. Maltose- 4B has also been used to study numerous pathological mechanisms for diseases in the human lung, including Aspergillus fumigatus, a pathogenic fungus, and the human influenza A virus.

Technical Specifications:

• Ligand: Maltose
• Matrix: 4B (agarose beads, 4%)
• Particle size range: 52 – 165 µm.
• Molecular weight range: 6 x 10⁴ – 2 x 10⁷
• Matrix activation: Divinyl sulfone
• Matrix attachment: Hydroxyl
• Ligand density: ~20 μmol maltose / ml drained gel
• Binding capacity: ≥20 mg MBP / ml drained gel
• Flow specifications: 70 – 140 cm / h
• pH stability: 4 – 9
• Supplied as suspension in 0.01M phosphate buffer (pH 6.8), 0.15M NaCl, 0.02% thimerosal

Additional Information

Additional Information

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