The enzyme immunoassay for lidocaine is based on the competition between the lidocaine to be assayed and the Lidocine-enzyme conjugate, for binding to antibody directed against the lidocaine, coated onto microtiter wells. The intesity of the color developed is inversely proportional to the concentration of lodocaine in the sample. Therefore, those samples that contain the lidocaine will inhibit binding of the enzyme conjugate to the capture antibody resulting in less color than the negative control. The concentration is estimated by comparison with standard lidocaine solution.
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