Kanamycin is a aminoglycosidic antibiotic which inhibits growth of gm-positive, gm-negative bacteria and mycoplasmas in cell culture. Kanamycin or kanamycin A (I) is the major component of the antibiotic complex produced by certain strains of Streptomyces kanamyceticus. Kanamycin A is supplied in two forms, a crystalline monosulfate monohydrate and a salt with a higher sulfate content. Kanamycin sulfate, which is sometimes referred to as “kanamycin bisulfate,” is obtained by adding sulfuric acid to a solution of the monosulfate and drying using a suitable procedure. Kanamycin Sulfate binds to 30S ribosomes causing inhibition of bacterial protein synthesis.
Applications:
Lab Application - It is used as resistance marker to grow plasmids of interest in competent bacterial cells.
Therapeutic Use - Kanamycin as a second-line antituberculosis drug.
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