Heparin (Agarose) 4B

Applications:

Heparin 4B is an affinity matrix  widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid receptors. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. Heparin- exhibits excellent binding capacity, a high flow rate, and no significant loss of the heparin ligand. Heparin 4B contains heparin (from porcine intestinal mucosa) immobilized by CNBr activation coupling method.

Technical specifications:

• Ligand: Porcine heparin
• Matrix: 4B (agarose beads, 4%)
• Particle size range: 52 – 165 µm
• Molecular weight range: 6 x 10⁴ – 2 x 10⁷
• Ligand coupling method: CNBr activation
• Ligand density: 5mg heparin / ml gel
• Binding capacity: ~ 2 mg bovine antithrombin / ml gel
• pH stability: 2 – 11 (ligand dependent)
• Storage: 2 – 8 °C
• Supplied as suspension in 0.5 M NaCl

Additional Information

Additional Information

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