Epoxy activated (Agarose) 6B-CL

Highlights:

• Used in coupling of hydroxyl, amino or thiol groups to 6B-CL via a 12-carbon hydrophilic spacer arm.
• Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
• Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
• Epoxy-activated 4B-CL is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
• Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.

Technical specifications:

• Matrix active group: Epoxy
• Matrix: 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 – 165 μm
• Group to be coupled: -NH₂, -OH, or -SH
• Molecular weight range: 6 x 10⁴ – 2 x 10⁷
• Chemical linkage of ligand: Ether
• Matrix spacer: 12 atoms (when ligands are coupled to free epoxy groups. Linkage is stable, uncharged ether)
• Coupling conditions: pH 9 – 10 (amino ligands), pH 11 – 12 (hydroxyl ligands), pH 7.5 - 8.5 (thiol ligands); Temp: 4 – 37 °C; Time: 16 h to several days; organic solvents possible
• pH stability: 2 – 11 (ligand dependent)
• Storage: 2 – 8 °C
• Supplied as lyophilized powder stabilized with lactose and dextran
• 1g swells to 3 – 4 ml

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