EDTA, disodium salt, for molecular biology is extensively used as a chelator of divalent cations such as Ca2+. EDTA is a chelating agent, a general chemical, and a sequestrant. In molecular biology applications, addition of EDTA minimizes the impact of metal ion contaminants. Also used to facilitate enzymatic reactions that could be inhibited by heavy metal traces and it also inhibits enzymes, such as metalloproteases.
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