ECH (Agarose) 4B

Highlights:

• Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups.
• Ligands are coupled in a single-step procedure in the presence of a coupling reagent (not provided) which is a water-soluble carbodiimide, such as N-ethyl-N`-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC).
• The matrix provides a 10-atom spacer arm convenient for coupling of small ligands.
• No blocking reaction is necessary following the coupling reaction.
• Ideal for immobilization of small ligands stable in a low pH and containing primary amines.
• Coupling reaction can be carried out in organic solvent.

Technical specifications:

• Matrix active group: -COOH groups
• Matrix: 4B (agarose beads, 4%)
• Particle size range: 52 – 165 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Group to be coupled: -NH₂ groups
• Chemical linkage of ligand: Stable amide linkage
• Ligand density : 12 – 16 μmol carboxyl groups / ml drained matrix
• Spacer arm: 6-amino caproic acid, 10 atoms
• Coupling conditions pH 4.5 – 6, Temp: 4°C – 25 °C, Time: 1.5 – 24 h; organic solvents possible
• Storage: 2 – 8 °C

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