EAH (Agarose) 4B

Applications: EAH- 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), bio-World`s EAH-4B is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.

Technical specifications:

• Matrix active groups: -NH₂ groups
• Matrix: 4B (agarose beads, 4%)
• Particle size range: 52 – 165 μm
• Matrix activation: Epoxy
• Group to be coupled: -COOH groups
• Chemical linkage of ligand: Stable amide linkage
• Spacer arm: 6-amino caproic acid, 10 atoms
• Ligand density: 7 – 11 μmol amino / ml medium
• Coupling condition: pH 4.5 – 6, Temp: 4 – 25 °C
• Storage: 20% ethanol containing 0.9% NaCl

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Additional Information

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