INQUIRE for lead time from stock Active enzyme purified from E. coli. The rate of caspase enzymatic hydrolysis can be measured by release of AMC from the Ac-DEVD-AMC caspase substrate, emission at 440 nm excitation at 380 nm using a spectrofluorometer. Concentration: 5g Protein in 25l Buffer. Formulation: 5g Buffered in 50mM TRIS (pH 8.0) with 100mM Sodium Chloride and 50mM Imidazole.
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