Arachis hypogaea (Peanut) lectin (PNA) -Separopore®

Applications:

Arachis hypogaea (Peanut Agglutinin, PNA)  4B-CL is used for the purification of O-linked glycoproteins.

Peanut agglutinin is a 110,000 molecular weight lectin composed of four identical subunits of approximately 27,000 daltons each. PNA binds preferentially to a commonly occurring structure, galactosyl (β-1,3) N-acetylgalactosamine. This carbohydrate sequence (called the “T-antigen”) is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding has been employed as a measure of cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte human subpopulations and animals, and to measure the levels of lymphoid cell populations in many diseases. Presence of Ca⁺⁺ can enhance PNA binding.

Technical Specifications:

• Ligand source:  Arachis hypogaea (peanut Lectin).
• Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 µm
• Matrix activation: Cyanogen bromide
• Matrix attachment: Amino
• Ligand density: 5 mg Peanut lectin/ml drained gel.
• Binding Capacity: 0.5 – 1.5 mg of asialofetuin / ml of drained medium.
• Inhibiting/Eluting Sugar: 200 mM galactose.
• Carbohydrate for Elution: 0.1M lactose or 0.2M galactose in buffer. 10-50 mM N-acetylgalactosamine may also be used.
• Blood Group Specificity: T-antigen
• Form: 50:50 Liquid Suspension, PBS (1x) pH 7.4 w/ 0.01% Thimerosal
• pH stability: 4 – 9
• Application note: Calcium is required for binding. Ca²⁺ concentration should not exceed 0.5 mM in the buffer (to not form a white precipitate).

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Additional Information

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