AMP (Agarose) 4B-CL (Lyophilized)

Applications:

AMP interacts strongly with NAD⁺-dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD⁺ or NADP⁺ allows the resolution of complex mixtures of dehydrogenase isoenzymes using 5`-AMP Separopore^®.

The production method involves alkylation of the nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6-carboxymethyl derivative with subsequent condensation using 1,6-diaminohexane to give N6-.

Technical Specifications:

• Ligand: Adenosine Monophosphate (AMP)
• Matrix: Separopore® 4B-CL (highly crosslinked agarose beads, 4%).
• Particle size range: 52 – 165 µm
• Matrix activation: Epoxy
• Matrix attachment: N6
• Spacer arm: 1, 6-diaminohexane
• Ligand density: 2 µmol AMP / ml drained gel
• Binding capacity: ~ 5-7 mg lactate dehydrogenase / ml drained gel
• pH stability: 4 – 10
• Flow rate specifications: 70 – 140 cm / h.
• Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Supplied as lyophilized powder and 1g yields 8-10 ml of gel

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Additional Information

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