2-Chloro-4-nitrophenyl α-L-fucopyranoside (CNPF) is a substrate for the enzyme α-L-fucosidase (AFU). CNPF is hydrolysed by α-L-fucosidase to fucose and 2-chloro-4-nitrophenol. The levels of AFU in human serum are useful markers for diagnosing hepatocellular carcinoma (HCC), being significantly higher in patients with HCC than those with cirrhosis, other malignant neoplasms or control subjects. A highly sensitive and specific assay monitoring the hydrolysis of CNPF by AFU has been developed for use in the early diagnosis of HCC.
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