Anti-NGAL (rat) ABS 039-32

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产品名称: Anti-NGAL (rat) ABS 039-32

产品型号: ABS 039-32

产品展商: Bioporto

产品文档: 无相关文档

简单介绍

Anti-NGAL (rat)

Anti-NGAL (rat) ABS 039-32 的详细介绍

Cat. No.: ABS 039-32B
Product name: Anti-NGAL (rat)
Description: Mouse monoclonal antibody, biotinylated
Specificity: ABS 039-32 binds rat NGAL (neutrophil gelatinase-associated lipocalin)
Immunogen: Recombinant rat NGAL precursor
Application: ELISA
Species reactivity (positive): Rat
Species reactivity (negative): Human, pig, dog, mouse
Subclass: IgG1/k
Gene ID: 170496
Clone number: 32
Epitope: Not determined
Content: 50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification: Biotinylated
Form: Liquid
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Target: Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas.

Application 1: ELISA
ABS 039-32 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 039-15 (as capture antibody) for measuring rat NGAL,(measuring range 4-400 pg/mL in an optimized assay).

The figure illustrates the calibration curve of a sandwich assay with ABS 039-15 as the capture antibody and ABS 039-32B as the biotinylated detection antibody.

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A

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