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Infliximab(Remicade®) Pharmacokinetic ELISA

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产品名称: Infliximab(Remicade®) Pharmacokinetic ELISA
产品型号: EL-1611-091
产品展商: AffinityImmuno
产品文档: 无相关文档

简单介绍

Infliximab(Remicade®) Pharmacokinetic ELISA Catalog EL-1611-091 $599.99


Infliximab(Remicade®) Pharmacokinetic ELISA  的详细介绍
INTRODUCTION
Infliximab (Remicade®) is a chimeric monoclonal antibody against tumour necrosis factor alpha (TNF-α). Infliximab neutralizes the biological activity of TNF-α by binding with high affinity to the soluble (free floating in the blood) and transmembrane (located onthe outer membranes of T cells and similar immune cells) forms of TNF-α, and inhibits or prevents the effective binding of TNF-α with its receptors.
The Infliximab ELISA kit can be used for monitoring infliximab during clinical research and offers the scientists a tool for understanding of the safety and efficacy of the Infliximab and its biosimilars. The assay design utilizes a pair of antibodies that allows the detection of the whole Inflimximab molecule in biological matrices.
PRINCIPLE OF THE ASSAY
This assay employs the sandwich enzyme immunoassay technique. Anti- Infliximab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Infliximab present in biological matrices is bound by the immobilized anti- Infliximab antibody. After washing away any unbound substances, enzyme linked anti- Infliximab antibody is added to the wells. This antibody is developed and purified specifically against truncated Remicade® (domain residing in Fc portion of the Infliximab® molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Infliximab present in test samples. The color development is stopped and the intensity of the color is measured.

Infliximab(Remicade®) Pharmacokinetic ELISA 
Catalog EL-1611-091


Product Properties

DETECTION METHOD

Peroxidase / OD450  

STORAGE AND STABILITY

Stable at -20°C for 1 year 

SAFETY WARNINGS AND PRECAUTIONS

Research Use Only. Follow instructions. 

PRECISION

Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. 

DETECTION LIMIT

0.78ng/ml 

EACH KIT INCLUDES:

Coated microtiter plate, 96 wells 
Calibrator diluent. - 1.8ml 
Calibrator (200 ug/mL) - 12ul 
10X wash buffer - 25ml 
Assay buffer - 50ml 
1000X detection reagent - 17ul 
TMB - 12ml 
TMB stop solution - 12ml 
Plate sealers - 3  

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