For Research Use Only in the United States. Not for use in diagnostic procedures.
The MicroVue Osteocalcin immunoassay measures intact (de novo) osteocalcin in serum. Intact osteocalcin may be useful as a biochemical indicator of bone turnover.
Osteocalcin (OC) or BGP (bone gla protein) is found exclusively in bone tissue. It is a 5800 molecular weight extrahepatic vitamin K dependent protein produced by osteoblasts. It contains three gamma-carboxyglutamic acid residues which are thought to be involved in calcium ion and hydroxyapatite binding. It accounts for 10%-20% of the noncollagenous protein in bone. While the in vivo function of osteocalcin is unknown, its affinity for bone mineral constituents implies a role in bone formation.
A Highly Specific Assay for Intact OC
ELISA
96 wells/plate
Serum
Method: Competitive Analyte: Intact osteocalcin Specimen: Serum Specimen Volume: 25 µL LOD*: 0.45 ng/L LLOQ*: 2 ng/mL HLOQ*: 32 ng/L Precision CVs within-run: 5%-10% Precision CVs between-run: 5%-10% Incubation Time: 3.6 Hours Cross-reactivity: Intact OC 100% Reduced OC 0% N-, C- peptides 0%
*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
96-well microplate with reagents sufficient to test 40 samples in duplicate
Rabbit, Pig, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture, Guinea Pig No recognition of Rat, Mouse, or Dog
Add 25 µL Standards, Controls, and Samples Add 125 µL anti-Osteocalcin Antibody Incubate 2 hours at room temperature Wash 3 times with 1X Wash Buffer Add 150 µL reconstituted Enzyme Conjugate Incubate 60 minutes at room temperature Wash 3 times with 1X Wash Buffer Add 150 µL Working Substrate Solution Incubate 35-40 minutes at room temperature Add 50 µL Stop Solution Read Optical Density at 405 nm Data Reduction: 4 Parameter
ELISA Kit
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