Catalogue number
ANT-460
Synonyms
PDZ Domain Containing 1, PDZ-Containing Kidney Protein 1, Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF3, Na/Pi Cotransporter C-Terminal-Associated Protein 1, Sodium-Hydrogen Exchanger Regulatory Factor 3, CFTR-Associated Protein Of 70 KDa, NHERF-3, CAP70, PDZD1, CLAMP, naPi-Cap1.
Type
Mouse Anti Human Monoclonal.
Introduction
PDZK1 is a PDZ domain-containing scaffolding protein which facilitates localization of cell surface proteins and has a key part in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphism in this gene is linked to metabolic syndrome, and overexpression results in drug resistance of multiple myeloma.
Clone
PAT1A2AT.
Immunogen
Anti-human PDZK1 mAb, clone PAT1A2A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDZK1 protein 1-519 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and Kappa light chain.
Purification Method
PDZK1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
