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ProSpecBio
MHC Class II Antibody
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产品名称:
MHC Class II Antibody
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简单介绍
MHC Class II
Antibody
MHC Class II Antibody
的详细介绍
Catalogue number
ANT-296
Type
Mouse Anti Human Monoclonal.
Introduction
MHC Class II is consists of 2 membrane spanning proteins. Each of these proteins is roughly 30 kDa, and has of two Globular domains, Alpha-1, Alpha-2, Beta-1 and Beta-2. The two regions farthest away from the membrane are alpha-1 and beta-1. The two proteins link without Covalent Bonds. The MHC Class II protein mainly presents peptides, which have been digested from external sources. MHC Class II is normally only expressed by Antigen Presenting Cells (APC) which digest foreign protein. Within the RER the alpha and beta proteins of the molecule, associate with each other, while a third protein called the "invariant chain," is necessary for stabilizing the complex, without it, they will not associate. The MHC-invariant complex is then passed from the RER, into and out of, the Golgi body. It fuses with an Endocytic compartment, where an external protein has been sampled and degraded.
Clone
CYR-hClass II.
Immunogen
Purified Human B cells.
Ig Subclass
Mouse IgG2a.
Specificity
All haplotypes.
Purification Method
Ion exchange column.
Formulation
1mg/ml in PBS (after reconstitution).
Shipping Conditions
Antibody
is shipped lyophilized at ambient temperature.
Storage Procedures
Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Solubility
Reconstitute with sterile H20 to give a 1mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Titer
Staining antibody. For staining, use 10µl/1,000,000 cells.
Applications
FACS staining, Western Blot, Immuneprecipitation.
Available Conjugates
This antibody is currently available only as purified antibody.
Safety Data Sheet
SDS
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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