LY6G Antibody

Rat Anti Mouse Monoclonal.

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g, Gr1, Gr-1.

Myeloid differentiation antigen Gr1 (Ly6G) is a GPI-anchored protein, which is briefly expressed on monocytes in the bone marrow. The level of the Ly6G expression in the bone marrow completely correlates with granulocyte differentiation and maturation. Ly6G ligation on murine neutrophils inhibits neutrophil recruitment, thus providing the first evidence of a function for the Ly6G molecule. Ly6G is seen primarily on neutrophils, also in a subgroup of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells.

YRmLy-6G.

Ly-6G-transfected cell line.

Rat IgG2a.

Protein A column.

1mg/ml in PBS (after reconstitution).

Antibody is shipped lyophilized at ambient temperature.

In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Flow cytometry, immunohistochemistry, cell depletion.
This antibody recognizes only Ly-6G which is expressed on most myeloid cells in the bone marrow and all peripheral granulocytes. For flow cytometry, use 10 µl/10⁶ cells. Exact titer for cell depletion in vivo (cytotoxicity) should be determined by the investigator.

This antibody is also available conjugated to biotin and FITC.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.