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ProSpecBio
Acrp30 Mouse, HEK
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产品名称:
Acrp30 Mouse, HEK
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简单介绍
Acrp30 Mouse, HEK
Acrp30 Mouse, HEK
的详细介绍
Catalogue number
CYT-435
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Introduction
Adiponectin is an important negative regulator in hematopoiesis and immune systems. It may be involved in ending inflammatory responses through its inhibitory functions. Inhibits endothelial nf-kappa-b signaling through a camp-dependent pathway. Inhibits tnf-alpha- induced expression of endothelial adhesion molecules. Involved in the control of fat metabolism and insulin sensitivity.
Description
The Acrp30 Mouse Recombinant is fused with FLAG tag having a total Mw of 26kDa.
Source
HEK293 (Human embryonic kidney cell line).
Purity
Purity of adiponectin mouse recombinant is >98% (HPLC and SDS PAGE analyzed).
Biological Activity
Full-length mouse adiponectin activates AMP-activated protein kinase in hepatocyte and activates AMPK in HepG2 human hepatocytes at a concentration of 1000ng/ml corresponding to a Specific Activity of 1000IU/mg. Mouse adiponectin mammalian cell derived inhibits glucose production as shown by in-vitro gluconeogenesis assay in primary rat hepatocytes.
Formulation
Filtered (0.4µm) and lyophilized from 0.5 mg/ml in PBS buffer.
Solubility
It is recommended to reconstitute the lyophilized Acrp30 Mouse in sterile 18M-cm H
2
O at 0.5mg/ml, which can then be further diluted to other aqueous solutions.
Stability
Store lyophilized APM-1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Amino acid sequence
EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK.
Safety Data Sheet
SDS
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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