Cyclin-Dependent Kinase 1 Human Recombinant, Sf9

Introduction: Cyclin-dependent kinase 1 (CDK1) plays a significant part in the control of the eukaryotic cell cycle through modulating the centrosome cycle in addition to mitotic onset; CDK1 promotes G2-M transition and regulates G1 progress and G1-S transition using association with multiple interphase cyclins. CDK1 is essential in higher cells for the entry into S-phase and mitosis.

Description: CDK1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 305 amino acids (1-297a.a.) and having a molecular mass of 35.1kDa.
CDK1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Physical Appearance: Sterile filtered colorless solution.

Formulation:
CDK1 protein solution (0.5mg/ml) contains Phosphate buffered saline (pH7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF.

Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid multiple freeze-thaw cycles.