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VGAT lumenal domain - 131 103CpH

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产品名称: VGAT lumenal domain - 131 103CpH
产品型号: 131 103CpH
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VGAT lumenal domain - 131 103CpH


VGAT lumenal domain - 131 103CpH  的详细介绍
Cat. No. 131 103CpH 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 200 gallery  
IHC: yes
IHC-P/FFPE: not tested yet
Label CypHer5E
Immunogen Synthetic peptide corresponding to AA 510 to 525 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity Specific for VGAT. (K.O. verified)
Remarks

This antibody is intended for direct labeling of recycling synaptic vesicles at inhibitory nerve terminals in living primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. Further details see Martens et al. 2008.
Datasheet 131_103cph.pdf
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