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Synaptotagmin 1 lumenal domain - 105 103CpH

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产品名称: Synaptotagmin 1 lumenal domain - 105 103CpH
产品型号: 105 103CpH
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Synaptotagmin 1 lumenal domain - 105 103CpH


Synaptotagmin 1 lumenal domain - 105 103CpH  的详细介绍
Cat. No. 105 103CpH 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A (see remarks)
IP: N/A
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label CypHer5E
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707), mouse (P46096).
Other species not tested yet.
For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Specificity Specific for synaptotagmin 1, no cross-reactivity to synaptotagmin 2.
matching control protein/peptide 105-10P
Remarks This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

WB: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.
Datasheet 105_103cph.pdf
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