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Synaptotagmin 1 cytoplasmic tail - 105 011

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产品名称: Synaptotagmin 1 cytoplasmic tail - 105 011
产品型号: 105 011
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Synaptotagmin 1 cytoplasmic tail - 105 011


Synaptotagmin 1 cytoplasmic tail - 105 011  的详细介绍
Cat. No. 105 011 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
EM: yes
ELISA: yes (see remarks)
Clone 41.1
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop Epitop: AA 150 to 240 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: human (P21579), rat (P21707), mouse (P46096), mammals, zebrafish.
Other species not tested yet.
Specificity Specific for mammalian synaptotagmin 1, no cross-reactivity to other synaptotagmins. (K.O. verified)
Remarks

WB: Use only non-boiled samples when running SDS-PAGE.

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 105 002 as detector antibody (protocol for sandwich-ELISA).
Since synaptotagmin 1 is unevenly expressed in neuronal subpopulations and may, in fact, be missing from some, it is less well suited as a general marker for synapses.
Datasheet 105_011.pdf
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